![]() © 2017 Wiley Periodicals, Inc.įc-fusion protein concentration polarization intermolecular interactions monoclonal antibody physical properties ultrafiltration viscosity. mAb monoclonal antibody, pCO2 partial pressure of carbon dioxide. These results provide important insights into the factors controlling the filtrate flux during the UF of concentrated protein solutions and an effective framework for the design/analysis of UF processes for the formulation of antibody-based therapeutics. Figure 1: Flow diagram of upstream unit operations for manufacturing scenarios. The filtrate flux data were analyzed using a recently developed model that accounts for the effects of intermolecular interactions and transmembrane pressure gradients on the extent of concentration polarization. monoclonal antibody reagents in drug manufacturing A major use of mAb reagents is in the purification of drug substance by mAbs attached to a solid support (e.g., immunoaffinity chromatography). The maximum achievable protein concentration was directly correlated with the solution viscosity, which controls the pressure drop and extent of back-filtration in the cassette. ![]() Independent experimental measurements were performed to evaluate the protein osmotic pressure and solution viscosity. Filtrate flux data were obtained using a Pellicon 3 tangential flow filtration cassette over a wide range of transmembrane pressures and bulk protein concentrations. Flow diagram key: Reference these numbers in the product selection guide tool. recombinant antibodies vs traditional antibodies. ![]() The objective of this study was to quantitatively compare the filtrate flux behavior for two highly purified mAbs and an Fc-fusion protein under identical flow and buffer conditions. Find out more about our recombinant antibodies and production process here. Ultrafiltration (UF) is used for the final concentration and formulation of essentially all antibody-based therapeutics including both monoclonal antibodies (mAbs) and Fc-fusion proteins. ![]()
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